专利名称：METHODS FOR CULTURE AND
PRODUCTION OF SINGLE CELL
POPULATIONS OF HUMAN EMBRYONICSTEM CELLS (HESCS)
发明人：Alexey Terskikh,Ruchi Bajpai申请号：US14554045申请日：20141126
公开号：US20150152380A1公开日：20150604
摘要：We used ACCUTASE®, a commercially available cell detachment solution, forsingle cell propagation of pluripotent hESCs. Unlike trypsin dissociation, ACCUTASE®treatment does not significantly affect the plating efficiency of hESC dissociation intosingle cells. Cultures dissociated with ACCUTASE® to single cells at each passagemaintain a higher proportion of pluripotent cells as compared to collagenase-passagedhESCs. ACCUTASE®-treated hESCs can be grown to a high density as monolayers, and yetretain their pluripotency.
申请人：BURNHAM INSTITUTE FOR MEDICAL RESEARCH
地址：La Jolla CA US
国籍：US
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