RNA DETECTION METHOD AND DETECTION KIT

专利名称：RNA DETECTION METHOD AND DETECTION

KIT

发明人：FUJIKAWA, Toshihiko,OSAWA, Masako申请号：EP13790416.5申请日：20130513公开号：EP2851425A1公开日：20150325

专利附图：

摘要：Provided are a method and kit for detecting RNA, which allow RNA such asmicroRNA and messenger RNA to be detected with high sensitivity and simply, and signalamplification probes to be used in the method. Specifically, provided is a method of

detecting target RNA, the method including: (A) a capturing step of capturing RNA by acapture substance for capturing the RNA; (B) a complex-for-detection forming step ofsubjecting the captured RNA and signal amplification probes capable of self-assemblingto a reaction to form a complex for detection including the RNA, the capture substance,and a probe polymer formed from the signal amplification probes; and (C) a detectingstep of detecting the probe polymer bound in the complex for detection to detect theRNA, one of the signal amplification probes including a poly(T) sequence.

申请人：Eisai R&D Management Co., Ltd.

地址：6-10, Koishikawa 4-chome Bunkyo-ku Tokyo 112-8088 JP

国籍：JP

代理机构：Grünecker, Kinkeldey, Stockmair & Schwanhäusser

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