Sequencing process

专利名称：Sequencing process

发明人：Burke, Catherine Maree,Darling, Aaron Earl申请号：AU2015263055申请日：20150522公开号：AU2015263055B2公开日：20201203

摘要：The present invention relates to methods for generating sequences oftemplate nucleic acid molecules, methods for determining sequences of at least twotemplate nucleic acid molecules, computer programs adapted to perform the methodsand computer readable media storing the computer programs. In particular the presentinvention relates to methods for generating sequences of at least one individual targettemplate nucleic acid molecule comprising: a) providing at least one sample of nucleicacid molecules comprising at least two target template nucleic acid molecules; b)introducing a first molecular tag into one end of each of the at least two target templatenucleic acid molecules and a second molecular tag into the other end of each of the atleast two target template nucleic acid molecules to provide at least two taggedtemplate nucleic acid molecules wherein each of the at least two tagged templatenucleic acid molecules is tagged with a unique first molecular tag and a unique secondmolecular tag; c) amplifying the at least two tagged template nucleic acid molecules toprovide multiple copies of the at least two tagged template nucleic acid moleculescomprising the first molecular tag and the second molecular tag; d) sequencing regionsof the at least two tagged template nucleic acid molecules comprising the first moleculartag and the second molecular tag; and e) reconstructing a consensus sequence for at

least one of the at least two target template nucleic acid molecules.

申请人：Longas Technologies Pty Ltd

代理人：Spruson & Ferguson

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