Apparatus for and method of purifying nucleic acid

专利名称：Apparatus for and method of purifying

nucleic acids by using beads with differentlaser absorption

发明人：Lee, Jeong-gun,Kwon, Young-nam 104-306,Lee, Myo-yong 306-1102 LG Xi Apt.,Yoo,Shin-i 101-2102 Ssangyong Apt.,Cho, Yeon-ja1-132 Jeonnong 3-dong,Kim, Young-a 245-1804

申请号：EP05015561.3申请日：20050718公开号：EP1662008B1公开日：20091216

摘要：An apparatus for and method of purifying nucleic acids of cells or viruses areprovided. The nucleic acid purification apparatus includes: a cell lysis capillary having asample inlet through which samples, magnetic beads, and a solid support are introduced;a vibrator attached to the capillary and mixing the samples, magnetic beads, and solidsupport in the capillary; a laser generator attached to the capillary and irradiating a laserbeam onto the capillary; a magnetic force generator attached to the capillary and fixingthe magnetic beads to a capillary wall; a waste chamber attached to the capillary anddischarging a lysate; an elution buffer chamber attached to the capillary and elutingnucleic acids from the solid support having nucleic acids bound thereto; and a

neutralization buffer chamber attached to the capillary and supplying a neutralizationbuffer for neutralizing the eluted nucleic acid solution. According to the apparatus and

method, PCR inhibitors can be removed to increase PCR yield and nucleic acids can bepurified using a silicon substrate or silica beads. Thus, the apparatus and method can beapplied to LOC fabrication.

申请人：SAMSUNG ELECTRONICS CO LTD

地址：KR

国籍：KR

代理机构：Grünecker, Kinkeldey, Stockmair & Schwanhäusser Anwaltssozietät

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