常用荧光染料的激发及发射波
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荧光素名称 1,5 IAEDANS 1,8-ANS 4-Methylumbelliferone 激发波长 (nm) 发射波长 (nm) 备注 336 490 372 385 480 502 529 518 563/668 568 518 520-540 520-540 604591 552568 543 543 548 430 647 449, 455 471, 474 445 630 Ratio Dye, pH 5-carboxy-2,7-dichlorofluorescein 504 5-Carboxyfluorescein (5-FAM) 492 5-Carboxynapthofluorescein (pH 512/598 10) 5-Carboxytetramethylrhodamine (5-542 TAMRA) 5-FAM (5-Carboxyfluorescein) 5-HAT (Hydroxy Tryptamine) 5-Hydroxy Tryptamine (HAT) 5-ROX (carboxy-X-rhodamine) 5-TAMRA (5-Carboxytetramethylrhodamine) 6-Carboxyrhodamine 6G 6-CR 6G 6-JOE 7-Amino-4-methylcoumarin 7-Aminoactinomycin D (7-AAD) 7-Hydroxy-4-methylcoumarin 9-Amino-6-chloro-2-methoxyacridine ABQ Acid Fuchsin 492 370-415 370-415 578567 548542 518 518 520 351 546 360 412, 430 344 540 2
ACMA (9-Amino-6-chloro-2-methoxyacridine) Acridine Orange + DNA Acridine Orange + RNA 412, 430 502 460 471, 474 526 650 520-650 560-680 550 520 460 466 Photoprotein Acridine Orange, both DNA & RNA 440-480 Acridine Red Acridine Yellow Acriflavin Acriflavin Feulgen SITSA Aequorin (Photoprotein) 455-600 470 436 355-425 AFPs - AutoFluorescent Protein - (Quantum Biotechnologies) see sgGFP, sgBFP Alexa Fluor 350? Alexa Fluor 430? Alexa Fluor 488? Alexa Fluor 532? Alexa Fluor 546? Alexa Fluor 568? Alexa Fluor 594? Alexa Fluor 633? Alexa Fluor 647? Alexa Fluor 660? Alexa Fluor 680? 346342 431 495, 492 531, 532 556, 557 577, 578 590, 594 632 647 668 679 442441 540 519, 520 553, 554 572, 573 603 617, 618 650 666 698 702 3
Alizarin Complexon Alizarin Red Allophycocyanin (APC) AMC, AMCA-S AMCA (Aminomethylcoumarin) AMCA-X Aminoactinomycin D Aminocoumarin Aminomethylcoumarin (AMCA) Anilin Blue Anthrocyl stearate APC (Allophycocyanin) APC-Cy7 APTRA-BTC = Ratio Dye, Zn2+ APTS Astrazon Brilliant Red 4G Astrazon Orange R Astrazon Red 6B Astrazon Yellow 7 GLL Atabrine ATTO-TAG CBQCA ATTO-TAG FQ Auramine 530-560, 580 580, 624-645 530-560 630, 645 345 345347 353 555 346350 345347 360-381 630, 645 625-650 466/380 424 500 470 520 450 436 465 486 460 580 655, 660 445 425444 442 655 442445 425444 600 446 655, 660 755 520/530 505 585 540 595 480 490 560 591 550 Ratio Dye, Zn2+ 4
Aurophosphine G Aurophosphine 450 450-490 580 515 395 520,528 520 550 447, 520 BAO 9 (Bisaminophenyloxadiazole) 365 BCECF (high pH) BCECF (low pH) Berberine Sulphate Beta Lactamase 492, 503 482 430 409 blue shifted GFP BFP blue shifted GFP (Y66H)Blue (Y66H)Blue 381, 382, 383 445, 447, 448 Fluorescent Protein Fluorescent Protein BFP/GFP FRET Bimane Bisbenzamide Bisbenzimide (Hoechst) bis-BTC = Ratio Dye, Zn2+ Blancophor FFG Blancophor SV BOBO -1 BOBO -3 Bodipy 492/515 Bodipy 493/503 Bodipy 500/510 Bodipy 505/515 398 360 360 455/405 390 370 462 570 490 533 509 502 490 461 461 529/505 470 435 481 602 515 549 515 510 Ratio Dye, Zn2+ 5
Bodipy 530/550 Bodipy 542/563 Bodipy 558/568 Bodipy 564/570 Bodipy 576/589 Bodipy 581/591 Bodipy 630/650-X Bodipy 650/665-X Bodipy 665/676 Bodipy Fl Bodipy FL ATP Bodipy Fl-Ceramide Bodipy R6G SE Bodipy TMR Bodipy TMR-X conjugate Bodipy TMR-X, SE Bodipy TR Bodipy TR ATP Bodipy TR-X SE BO-PRO -1 BO-PRO -3 Brilliant Sulphoflavin FF BTC - Ratio Dye Ca2+ 528 543 558 564 579 584 625 647 605 504, 505 505 505 528 542 544 544 589 591 588 462 544 430 464/401 547 563 569 570 590 592 642 665 676 511, 513 514 511 547 574 573 570 617 620 616 481 570 520 533/529 Ratio Dye Ca2+ 6
BTC-5N - atio Dye, Zn2+ Calcein Calcein Blue Calcium Crimson? Calcium Green Calcium Green-1 Ca2+ Dye Calcium Green-2 Ca2+ Calcium Green-5N Ca2+ Calcium Green-C18 Ca2+ Calcium Orange Calcofluor White Carboxy-X-rhodamine (5-ROX) Cascade Blue? Cascade Yellow Catecholamine CCF2 (GeneBlazer) CFDA CFP - Cyan Fluorescent Protein CFP/YFP FRET Chlorophyll Chromomycin A Chromomycin A CL-NERF (Ratio Dye, pH) CMFDA Coelenterazine Ca2+ Dye, 459/417 494 373 588, 589 501, 506 506 506/503 506 509 549 517/532 517 440 611, 615 531 531 536 532 530 575576 Ratio Dye, Zn2+ Ca2+ Dye Ca2+ Ca2+ Ca2+ 385, 395, 405 437, 440, 445 576 377398399 399400 410 494 601 420423 550552 470 520 430, 433, 436, 474, 475, 476, Cyan Fluorescent (453) (501) Protein 480 436-460 445 504/514 494 (429) 650 470 575 540 520 465 Ratio Dye, pH Ca2+ Dye, 7
bioluminescence Coelenterazine cp (Ca2+ Dye,) Coelenterazine f Coelenterazine fcp Coelenterazine h Coelenterazine hcp Coelenterazine ip Coelenterazine n Coelenterazine O Coumarin Phalloidin C-phycocyanine CPM Methylcoumarin CTC CTC Formazan Cy2? Cy3.1 8 Cy3.5? Cy3? Cy5.1 8 Cy5.5? Cy5? (430) (437) (437) (433) (431) 460 387 384 400-450 489 554 581 514552554 649 675 649 442 473 452 464 444 441 467 575 470 469 602 506 568 598 566570 666 695 666670 bioluminescence, native molecule Ca2+ Dye, bioluminescence Ca2+ Dye, bioluminescence Ca2+ Dye, bioluminescence Ca2+ Dye, bioluminescence Ca2+ Dye, bioluminescence Ca2+ Dye, bioluminescence Ca2+ Dye, bioluminescence Methylcoumarin 8
Cy7? Cyan GFP 710, 743 433 (453) 767, 805 475 (501) 517 520 578 517 518 518 517 none 461 580 574 574 529 535 607 534 705 605713 Cell Proliferation Assay cyclic AMP Fluorosensor (FiCRhR) 500 CyQuant Cell Proliferation Assay 480 Dabcyl Dansyl Dansyl Amine Dansyl Cadaverine Dansyl Chloride Dansyl DHPE Dansyl fluoride DAPI Dapoxyl Dapoxyl 2 Dapoxyl 3 DCFDA 453 340 337 335 372 336 356 359 403 374 373 504 DCFH (Dichlorodihydrofluorescein 505 Diacetate) DDAO DHR (Dihydorhodamine 123) Di-4-ANEPPS Di-8-ANEPPS (non-ratio) 463 505 496 488498 9
DiA (4-Di-16-ASP) Dichlorodihydrofluorescein Diacetate (DCFH) DiD - Lipophilic Tracer DiD (DiIC18(5)) DIDS Dihydorhodamine 123 (DHR) DiI (DiIC18(3)) Dinitrophenol DiO (DiOC18(3)) DiR DiR (DiIC18(7)) DM-NERF (high pH) DNP Dopamine DsRed DTAF DY-630-NHS 456 505 644 644 341 505 549, 551 349 484, 487 748 750 497/510 349 340 558 494 621 591 535 665 665 415 535 565 501, 502 780 779 540 490-520 583 520 660 Lipophilic Tracer Lipophilic Tracer Ratio Dye, pH Red fluorescent protein Hemicyanelabel for proteins and DNA Hemicyanelabel for proteins and DNA Enhanced Blue Fluorescent Protein Enhanced Cyan Fluorescent DY-635-NHS 634 664 EBFP ECFP 383 436 447 474 10
EGFP ELF 97 Eosin Erythrosin Erythrosin ITC Ethidium Bromide Ethidium homodimer -1 (EthD-1) Euchrysin EukoLight Europium (III) chloride EYFP Fast Blue FDA Feulgen (Pararosaniline) FIF (Formaldehyd Induced Fluorescence) FITC FITC Antibody Flazo Orange Fluo-3 Fluo-4 488, 498 345 524 529, 532 529 510, 523 528 430 507, 516 530 545 554, 555 555 595, 605 617 540 Protein Enhanced Green Fluorescent Protein Enhanced Yellow Fluorescent Protein 513, 520 360 494 570 405 490, 494 493 375-530 480 – 506, 506 494 527, 532 440 520 625 433 520, 525 517 612 520, 527 516 11
Fluorescein (FITC) Fluorescein Diacetate Fluoro-Emerald 490, 494 494 495 520, 525 520 524 536 582 520 598 640 657 505 512 590 485 583 475 516 516 509 509 Excitation ratio dye Excitation ratio dye Fluoro-Gold (Hydroxystilbamidine) 361 Fluor-Ruby FluorX FM 1-43? FM 4-46 Fura Red (high pH) Fura Red/Fluo-3 Fura-2, high calcium Fura-2, low calcium Fura-2/BCECF Genacryl Brilliant Red B Genacryl Brilliant Yellow 10GF Genacryl Pink 3G Genacryl Yellow 5GF GeneBlazer (CCF2) GFP (S65T) GFP red shifted (rsGFP) 555 494 479 515 572 335 363 520 430 470 430 498 498 GFP wild type, non-UV excitation 475 (wtGFP) GFP wild type, UV excitation 395 (wtGFP) 12
GFPuv Gloxalic Acid Granular Blue Haematoporphyrin Hoechst 33258 Hoechst 33342 Hoechst 34580 HPTS Hydroxycoumarin 385 405 355 530-560 345 347 392 355 325-360 508 460 425 580 487 483 440 465 386-455 536 530 401 475 665 780 430 529 545 544 812 460 712 Emission ratio dye Emission ratio dye Hydroxystilbamidine (FluoroGold) 361 Hydroxytryptamine Indo-1, high calcium Indo-1, low calcium Indodicarbocyanine (DiD) Indotricarbocyanine (DiR) Intrawhite Cf JC-1 JO-JO-1 JO-PRO-1 LaserPro Laurodan LDS 751 (DNA) 400 330 346 644 748 360 514 530 532 795 355 543 13
LDS 751 (RNA) Leucophor PAF Leucophor SF Leucophor WS Lissamine Rhodamine Lissamine Rhodamine B 590 370 380 395 572, 577 577 607 430 465 465 591, 592 592 517617 580 581 528, 536, 540 422 536 511, 551 516 576 424 505 540 531 600 659/631 for more details referto Ratio Dye, Mg2+ Ratio Dye Ca2+Ratio Dye Mg2+ LIVE/DEAD Kit Animal 494528 CellsCalcein/Ethidium homodimer LOLO-1 LO-PRO-1 Lucifer Yellow Lyso Tracker Blue Lyso Tracker Blue-White Lyso Tracker Green Lyso Tracker Red Lyso Tracker Yellow LysoSensor Blue LysoSensor Green LysoSensor Yellow/Blue Mag Green Magdala Red (Phloxin B) Mag-Fura Red Mag-Fura-2 566 568 425, 428 373 466 504, 534 490 551 374 442 384 507 524 483/427 369/329369/330 508511/491 14
Mag-Fura-5 Mag-Indo-1 Magnesium Green Magnesium Orange Malachite Green Marina Blue Ratio Dye, 369/330369/332 505/500505/482 Ca2+Ratio Dye, Mg2+ Ratio Dye, 349/328349/330 480/390480/417 Ca2+Ratio Dye, Mg2+ 506, 507 531 550 628 362 575 459 495 495 578 410 516 576 599 470 490 500 461 395 539 530 Maxilon Brilliant Flavin 10 GFF 450 Maxilon Brilliant Flavin 8 GFF Merocyanin Methoxycoumarin Mitotracker Green FM Mitotracker Orange Mitotracker Red Mitramycin Monobromobimane Monobromobimane (mBBr-GSH) Monochlorobimane MPS (Methyl Green Pyronine Stilbene) NBD NBD Amine Nile Red 460 555 360 490 551 578 450 398 398 380 364 466 450 515-555, 559 590, 640 15
Nitrobenzoxadidole Noradrenaline Nuclear Fast Red Nuclear Yellow Nylosan Brilliant Iavin E8G Oregon Green Oregon Green 488-X Oregon Green? Oregon Green 488 Oregon Green 500 Oregon Green 514 Pacific Blue Pararosaniline (Feulgen) PBFI PE-Cy5 PE-Cy7 PerCP PerCP-Cy5.5 PE-TexasRed [Red 613] Phloxin B (Magdala Red) 465 340 289-530 365 460 503 494 503 490, 493 510-650 490-520 580 495 510 522 517 522 514, 520 497 517 506 405 570 340/380 488 488 488 488 488 524 526 455 625 420 670 755, 767 675 710 613 600 Excitation ratio dye 16
Phorwite AR Phorwite BKL Phorwite Rev Phorwite RPA Phosphine 3R PhotoResist Phycoerythrin B [PE] Phycoerythrin R [PE] PKH26 (Sigma) PKH67 PMIA Pontochrome Blue Black POPO-1 POPO-3 PO-PRO-1 PO-PRO-3 Primuline Procion Yellow Propidium Iodid (PI) PyMPO Pyrene Pyronine Pyronine B 360 370 380 375 465 365 546-565 565 551 496 341 535-553 433 533 435 539 410 470 (305), 536, 538 412, 415 360 410 540-590 430 430 430 430 565 610 575 578 567 520 376 605 457 574 455 567 550 600 617 561, 564, 570 387 540 560-650 Chroma 17
Pyrozal Brilliant Flavin 7GF QSY 7 Quinacrine Mustard Red 613 [PE-TexasRed] Resorufin RH 414 Rhod-2 Rhodamine Rhodamine 110 Rhodamine 123 Rhodamine 5 GLD Rhodamine 6G Rhodamine B Rhodamine B 200 Rhodamine B extra Rhodamine BB Rhodamine BG Rhodamine Green Rhodamine Phallicidine Rhodamine Phalloidine Rhodamine Red Rhodamine WT 365 560 440 488 571 532 552 550 496, 497 507 470 525 540 523-557 550 540 540 502 558, 542 542 570 530 495 510 613 584, 585 716 576 573 520 529 565 555 625 595 605 580 572 527 575, 565 565 590 555 18
Rose Bengal R-phycocyanine R-phycoerythrin (PE) rsGFP S65A S65C S65L S65T Sapphire GFP SBFI Serotonin Sevron Brilliant Red 2B Sevron Brilliant Red 4G Sevron Brilliant Red B Sevron Orange Sevron Yellow L sgBFP? sgBFP (super glow BFP) sgGFP? sgGFP (super glow GFP) SITS SITS (Primuline) 525, 540 565 473 471 479 484 488 395 340/380 365 520 500 530 440 430 387 387 474 474 336 395-425 550-600 578 509 504 507 510 511 511 420 520-540 595 583 590 530 490 450 450 488 509 436 450 red shifted GFP (S65T) Excitation ratio dye Quantum’s SuperGlo?GFPAFPs Quantum’s SuperGlo?GFPAFPs Ion Channels 19
SITS (Stilbene Isothiosulphonic Acid) SNAFL calcein SNAFL-1 SNAFL-2 SNARF calcein SNARF1 Sodium Green SpectrumAqua SpectrumGreen SpectrumOrange Spectrum Red SPQ (6-methoxy-N-(3-sulfopropyl)quinolinium) Stilbene Sulphorhodamine B can C Sulphorhodamine G Extra SYTO 11 SYTO 12 SYTO 13 SYTO 14 SYTO 15 SYTO 16 SYTO 17 SYTO 18 SYTO 20 SYTO 21 SYTO 22 SYTO 23 SYTO 24 SYTO 25 SYTO 40 SYTO 41 365 506/535 508/540 514/543 552/574 460 535/620 543/623 546/630 590/629 Ratio Dye, pH Ratio Dye, pH Ratio Dye, pH Ratio Dye, pH Excitation and 576/548 635/587 emission ratio dye 506, 507 532 Na+, K+ 433,/53 480/55 Vysis 497/30, 509/31 538/44, 524/56 Vysis 559/38, 560 588/48 Vysis 587, 587/35 612, 612/51 344 335 520 470 508, 510 499, 500 488, 491 517, 521 516, 518 488, 494 621 490, 493 512 494 515 499 490 521 420 430 443 440 595 570 527, 530 522, 519 509, 514 549, 547 546, 555 518, 525 634 507, 527 530 517 535 520 515 556 441 454 Dye for DNA, RNA Dye for DNA, RNA Dye for DNA, RNA Dye for DNA, RNA Dye for DNA, RNA Dye for DNA, RNA Dye for DNA Dye for DNA, RNA Dye for DNA Dye for DNA Dye for DNA Dye for DNA Dye for DNA Dye for DNA Dye for DNA Dye for DNA 20
SYTO 42 SYTO 43 SYTO 44 SYTO 45 SYTO 59 SYTO 60 SYTO 61 SYTO 62 SYTO 63 SYTO 64 SYTO 80 SYTO 81 SYTO 82 SYTO 83 SYTO 84 SYTO 85 SYTOX Blue SYTOX Green SYTOX Orange Tetracycline Tetramethylrhodamine (TRITC) Texas Red? Texas Red-X conjugate Thiadicarbocyanine (DiSC3) Thiazine Red R 433 436 446 452 622 652 628 652 657 599 531 530 541 543 567 567 445 504 547 390-425 555 595 595 651, 653 596 460 467 471 484 645 678 645 676 673 619 545 544 560 559 582 583 470 523 570 525-560 576 620 615 674, 675 615 Dye for DNA Dye for DNA Dye for DNA Dye for DNA Dye for DNA Dye for DNA Dye for DNA Dye for DNA Dye for DNA Dye for DNA Nucleic Acid Stain Nucleic Acid Stain Nucleic Acid Stain Nucleic Acid Stain Nucleic Acid Stain Nucleic Acid Stain Nucleic Acid Stain Nucleic Acid Stain Nucleic Acid Stain 21
Thiazole Orange Thioflavin 5 Thioflavin S Thioflavin TCN Thiolyte Thiozole Orange Tinopol CBS (Calcofluor White) TMR TO-PRO-1 TO-PRO-3 TO-PRO-5 TOTO-1 TOTO-3 TriColor (PE-Cy5) 510 430 430 350 370-385 453 390 550 515 644 747 514 642 (488) 650 530 550 550 460 477-488 480 430 573 531 657 770 531, 533 660 667 573 425 695 678 520 435 508 639 605 wild type GFP TRITC 550 TetramethylRodamineIsoThioCyanate True Blue TruRed Ultralite Uranine B Uvitex SFC wt GFP WW 781 X-Rhodamine 365 490 656 420 365 395 (475) 605 580 22
XRITC Xylene Orange Y66F Y66H Y66W 582 546 360 360 436 601 580 508 442 485 Yellow shifted Green Fluorescent Protein Yellow Fluorescent Protein Yellow GFP 513 527 YFP YO-PRO-1 YO-PRO-3 YOYO-1 YOYO-3 常用荧光染料的激发及发射波长
513, 520 491 613 491 612 527, 532 506 629 508, 509 631 FluorescentDye（荧光染料）Excitation（激发波长nm）Emission发射波长nm） Cy2TM489506
GFP(RedShifted)488507 YO-PROTM-1491509 YOYOTM-1491509 Calcein494517 FITC494518 FluorXTM494519 AlexaTM488490520 Rhodamine110496520 ABI,5-FAM494522 OregonGreenTM500503522 OregonGreenTM488496524 RlboGreenTM500525
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RhodamineGreenTM502527 Rhodamine123507529 MagnesiumGreenTM506531 CalciumGreenTM506533 TO-PROTM-1514533 TOTO-1514533 ABI,JOE520548 BODIPY530/550530550 Dil549565 BODIPYTMR542568 BODIPY558/568558568 BODIPY564/570564570 Cy3TM550570 AlexaTM546555570 TRITC547572
MagnesiumOrangeTM550575 Phycoerythrin,RB565575 RhodaminePhalloidin550575CalciumOrangeTM549576 PyroninY555580 RhodamineB555580 ABI,TAMRA560582 RhodamineRedTM570590 Cy3.5TM581596 ABI,ROX588608
CalciumCrimsonTM590615 AlexaTM594590615 TexasRed595615 NileRed549628 YO-PROTM-3612631 YOYOTM-3612631 R-Phycocyanin618642 C-Phycocyanin620648 TO-PROTM-3642660 TOTO-3642660
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DiD?DilC’(5)644665 Cy5TM649670
Thiadicarbocyanine651671 Cy5.5675694
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